[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND OPTIMAL PH. STRAIN=Nine Mile I; DOI=10.1111/j.1574-6968.1999.tb13607.x; PubMed=10361714 [NCBI, ExPASy, EBI, Israel, Japan] Van Nguyen S., To H., Yamaguchi T., Fukushi H., Hirai K.; "Molecular cloning of an immunogenic and acid-induced isocitrate dehydrogenase gene from Coxiella burnetii."; FEMS Microbiol. Lett. 175:101-106(1999).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=1M, 27M, 307, 3M, 605, 60M, 82M, Bangui VR 730, El Tayeb RSA 342, GQ212, KoQ229, MAN, ME, Ohio 314 VR 542, Priscilla, SQ217, and TK-1; DOI=10.1111/j.1574-6968.1999.tb08803.x; PubMed=10556719 [NCBI, ExPASy, EBI, Israel, Japan] Nguyen S.V., Hirai K.; "Differentiation of Coxiella burnetii isolates by sequence determination and PCR-restriction fragment length polymorphism analysis of isocitrate dehydrogenase gene."; FEMS Microbiol. Lett. 180:249-254(1999).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=Nine Mile phase I / RSA 493; DOI=10.1073/pnas.0931379100; PubMed=12704232 [NCBI, ExPASy, EBI, Israel, Japan] Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A., Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F.; "Complete genome sequence of the Q-fever pathogen, Coxiella burnetii."; Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003).
[4]	IDENTIFICATION BY MASS SPECTROMETRY, AND DEVELOPMENTAL STAGE. STRAIN=Nine Mile Crazy / RSA 514; DOI=10.1128/IAI.00883-06; PubMed=17088354 [NCBI, ExPASy, EBI, Israel, Japan] Coleman S.A., Fischer E.R., Cockrell D.C., Voth D.E., Howe D., Mead D.J., Samuel J.E., Heinzen R.A.; "Proteome and antigen profiling of Coxiella burnetii developmental forms."; Infect. Immun. 75:290-298(2007).

Comments

CATALYTIC ACTIVITY: Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
CATALYTIC ACTIVITY: Oxalosuccinate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
BIOPHYSICOCHEMICAL PROPERTIES:

pH dependence: Optimum pH is 6.5-7.7;
DEVELOPMENTAL STAGE: Detected in both the small cell variant (SCV) and in the large cell variant (LCV) stage (at protein level). LCVs are more metabolically active than SCVs.
SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF069035; AAC68600.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146284; AAF16725.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146285; AAF16726.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146286; AAF16727.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146287; AAF16728.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146288; AAF16729.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146289; AAF16730.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146290; AAF16731.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146291; AAF16732.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146292; AAF16733.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146293; AAF16734.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146294; AAF16735.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146295; AAF16736.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146296; AAF16737.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146297; AAF16738.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146298; AAF16739.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146299; AAF16740.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146300; AAF16741.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF146301; AAF16742.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AE016828; AAO90709.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_820195.1; -.

3D structure databases

HSSP

P08200; 1PB1. [HSSP ENTRY / SWISS-3DIMAGE / PDB]

SMR

Q9ZH99; 15-426.

ModBase

Q9ZH99.

Enzyme and pathway databases

BioCyc

CBUR227377:CBU_1200-MON; -.

Ontologies

GO:0004450;	Molecular function: isocitrate dehydrogenase (NADP+) activity (inferred from electronic annotation from InterPro).
GO:0000287;	Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145;	Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006097;	Biological process: glyoxylate cycle (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006099;	Biological process: tricarboxylic acid cycle (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR004439; IsoCit_DHase_NADP_prok.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.718.10; IDH_IMDH; 1.

PANTHER

PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF1; NADP_IDH_prok; 1.

Pfam

PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00183; prok_nadp_idh; 1.

PROSITE

PS00470; IDH_IMDH; 1.

ProtoNet

Q9ZH99.

Genome annotation databases

GeneID

1209104; -.

GenomeReviews

AE016828_GR; CBU_1200.

KEGG

cbu:CBU_1200; -.

NMPDR

fig|227377.1.peg.1139; -.

TIGR

CBU_1200; -.

Phylogenomic databases

HOGENOM

Q9ZH99; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Phosphoprotein; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 427`	`427`	`Isocitrate dehydrogenase [NADP].`	`PRO_0000323713`
`NP_BIND`	`349 355`	`7`	`NADP (By similarity).`
`METAL`	`317 317`		`Magnesium or manganese (By similarity).`
`BINDING`	`114 114`		`NADP (By similarity).`
`BINDING`	`123 123`		`Substrate (By similarity).`
`BINDING`	`125 125`		`Substrate (By similarity).`
`BINDING`	`129 129`		`Substrate (By similarity).`
`BINDING`	`139 139`		`Substrate (By similarity).`
`BINDING`	`163 163`		`Substrate (By similarity).`
`BINDING`	`362 362`		`NADP; via amide nitrogen and carbonyl oxygen (By similarity).`
`BINDING`	`401 401`		`NADP (By similarity).`
`BINDING`	`405 405`		`NADP (By similarity).`
`SITE`	`170 170`	`1`	`Critical for catalysis (By similarity).`
`SITE`	`240 240`	`1`	`Critical for catalysis (By similarity).`
`MOD_RES`	`123 123`		`Phosphoserine (By similarity).`
`VARIANT`	`20 20`	`1`	`Q -> E (in strain: GQ212, KoQ229, SQ217).`
`VARIANT`	`32 32`	`1`	`E -> V (in strain: TK-1).`
`VARIANT`	`33 33`	`1`	`V -> A (in strain: GQ212).`
`VARIANT`	`52 52`	`1`	`A -> T (in strain: GQ212, KoQ229, MAN, ME, Priscilla, SQ217).`
`VARIANT`	`63 63`	`1`	`V -> A (in strain: ME).`
`VARIANT`	`87 87`	`1`	`V -> A (in strain: SQ217).`
`VARIANT`	`100 100`	`1`	`E -> G (in strain: California 76 VR 614).`
`VARIANT`	`104 104`	`1`	`E -> G (in strain: 605).`
`VARIANT`	`183 183`	`1`	`V -> M (in strain: Priscilla).`
`VARIANT`	`271 271`	`1`	`G -> R (in strain: Bangui VR 730).`
`VARIANT`	`306 306`	`1`	`Y -> C (in strain: Bangui VR 730).`
`VARIANT`	`379 379`	`1`	`W -> R (in strain: El Tayeb RSA 342).`
`VARIANT`	`415 415`	`1`	`T -> A (in strain: GQ212, KoQ229, SQ217).`
`VARIANT`	`419 419`	`1`	`G -> A (in strain: GQ212, KoQ229, SQ217).`

Sequence information

Length: 427 AA [This is the length of the unprocessed precursor]

Molecular weight: 46636 Da [This is the MW of the unprocessed precursor]

CRC64: E23462962C26C138 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTELTGVSIV TYQHIKVPSQ GEKITVNKAV LEVPDRPIIP FIEGDGIGID IAPVMKNVVD 

        70         80         90        100        110        120 
AAVEKSYAGK RKIEWMEIYA GEKATKVYGK DNWLPDETLE AIKEYQVAIK GPLTTPVGGG 

       130        140        150        160        170        180 
IRSLNVALRQ QLDLYVCLRP VRYFTGVPSP VKTPEKVNMV IFRENSEDIY AGIEWPAGSP 

       190        200        210        220        230        240 
EAVKLINFLQ NEMGVKKIRF PETAGIGIKP VSKEGTSRLV RRAIQYAIDN DRDSVTLVHK 

       250        260        270        280        290        300 
GNIMKFTEGA FKDWGYEVAV KEFGAKPLDG GPWHVFENPK TGQKITIKDV IADAFLQQIL 

       310        320        330        340        350        360 
LRPAEYSVIA TLNLNGDYIS DALAAEVGGI GIAPGANLSD TVGLFEATHG TAPKYAGQDK 

       370        380        390        400        410        420 
VNPGSLILSA EMMLRYLGWK EAADLVVQGI EGAIESKTVT YDFARLMTGA KEVSTSQFGK 


AIIKHIL

Q9ZH99 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools