NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Madrid E;
DOI=10.1038/24094; PubMed=9823893 [NCBI, ExPASy, EBI, Israel, Japan]
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.;
"The genome sequence of Rickettsia prowazekii and the origin of mitochondria.";
Nature 396:133-140(1998).

Comments

CATALYTIC ACTIVITY: (S)-malate + NADP⁺ = pyruvate + CO₂ + NADPH.
COFACTOR: Divalent metal cations. Prefers magnesium or manganese (By similarity).
SIMILARITY: In the N-terminal section; belongs to the malic enzymes family.
SIMILARITY: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ235271; CAA14832.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

F71694; F71694.

RefSeq

NP_220756.1; -.

3D structure databases

ModBase

Q9ZDF6.

Enzyme and pathway databases

BioCyc

RPRO272947:RP373-MON; -.

Ontologies

GO:0004473;	Molecular function: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR012188; ME_PTA.
IPR002505; PTA_PTB.
Graphical view of domain structure.

Pfam

PF00390; malic; 1.
PF03949; Malic_M; 1.
PF01515; PTA_PTB; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF036684; ME_PTA; 1.

PROSITE

PS00331; MALIC_ENZYMES; 1.

Genome annotation databases

GeneID

883450; -.

GenomeReviews

AJ235269_GR; RP373.

KEGG

rpr:RP373; -.

Phylogenomic databases

HOGENOM

Q9ZDF6; -.

Genome annotation databases

CMR

Q9ZDF6; RP373.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Metal-binding; Multifunctional enzyme; NADP; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 767`	`767`	`Probable NADP-dependent malic enzyme.`	`PRO_0000160247`
`REGION`	`1 430`	`430`	`Malic enzyme.`
`REGION`	`431 767`	`337`	`Phosphate acetyltransferase.`
`ACT_SITE`	`97 97`		`Proton acceptor (By similarity).`
`METAL`	`139 139`		`Divalent metal cation (By similarity).`
`METAL`	`140 140`		`Divalent metal cation (By similarity).`
`BINDING`	`165 165`		`NAD (By similarity).`
`BINDING`	`290 290`		`NAD (By similarity).`

Sequence information

Length: 767 AA [This is the length of the unprocessed precursor]

Molecular weight: 84253 Da [This is the MW of the unprocessed precursor]

CRC64: 6782D33C85E30A5E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDEMNKINYT EALEYHEKDK PGKIAITTTK SLVTQQDLSL AYSPGVAAPC LEISKNLEAV 

        70         80         90        100        110        120 
YKYTSRSNLV AVISNGTAVL GLGNLGAAAS KPVMEGKAVL FKKFADIDAI DLEVNTEDPI 

       130        140        150        160        170        180 
EFINAVKYLG YSFGGINLED IKAPECFLIE EKLKSLMDIP VFHDDQHGTA IITAAGLINA 

       190        200        210        220        230        240 
AYLTNRTLKD LKIVINGAGA AAIACIDLLI ALGVDKSKII LCDTKGVIYK GRTSGMNKWK 

       250        260        270        280        290        300 
ERYASDTKIR TLTESLNNAD VFIGLSVKGA VTKDMISKMA HKPIIFAMAN PDPEITPEDI 

       310        320        330        340        350        360 
KFVRDDAIIA TGRSDYNNQV NNVMGFPYIF RGALDVRAST INTEMKIAAA RAIADLARRP 

       370        380        390        400        410        420 
VPEEVYKAYS GRKMVFGNEY IIPVPFDPRL ITVVATAVAV AAIESGVARV KDFSIDKYKQ 

       430        440        450        460        470        480 
QLGSRLNPTA NYMNFLAEKI HNVPLKRIVF AEGEEEEVIS AALMMRDEKY GNPIIIGRVE 

       490        500        510        520        530        540 
RIEVTLKKIG KDISLAGIQI MNAALSDRLE QYTDYLYKRL QRKGYLYRDC AKLVKTDKNI 

       550        560        570        580        590        600 
FAACMVACGD GDALLTGVTK SYIDSLEDII KVISPKQNRR ILGYSIMIAK DHNIIIADNC 

       610        620        630        640        650        660 
ITEYPNSLEL AQIATQTAEI AKNMGITPRV ALIAFSTFGN SSQEKTVRIR EAVNILDNFS 

       670        680        690        700        710        720 
KDKKKLNGIK VDFEYDGEMS VKVALDHDLR KLYQFCRLSG SANVLIMPGL NSAAISTELL 

       730        740        750        760 
QKFSSNSFIG PITNGFAKPV QILPTTATAN EILKIATFAC VEAIKEV

Q9ZDF6 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools