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UniProtKB/Swiss-Prot entry Q9HYD5

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAO1_PSEAE
Primary accession number Q9HYD5
Secondary accession numbers None
Integrated into Swiss-Prot on December 6, 2005
Sequence was last modified on March 1, 2001 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 42)
Name and origin of the protein
Protein name NAD-dependent malic enzyme
Synonyms NAD-ME
EC 1.1.1.38
Gene name
Name: sfcA
OrderedLocusNames: PA3471
From
Pseudomonas aeruginosa [TaxID: 287] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
DOI=10.1038/35023079; PubMed=10984043 [NCBI, ExPASy, EBI, Israel, Japan]
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.";
Nature 406:959-964(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE004091; AAG06859.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D83211; D83211.
RefSeq NP_252161.1; -.
3D structure databases
HSSP P27443; 1O0S. [HSSP ENTRY / PDB]
ModBase Q9HYD5.
Enzyme and pathway databases
BioCyc PAER208964:PA3471-MON; -.
Organism-specific databases
PseudoCAP PA3471; -.
Ontologies
GO
GO:0016619; Molecular function: malate dehydrogenase (oxaloacetate-decarboxylating) activity (inferred from electronic annotation from HAMAP).
GO:0046872; Molecular function: metal ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051287; Molecular function: NAD binding (inferred from electronic annotation from InterPro).
GO:0006108; Biological process: malate metabolic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
HAMAP MF_01619; -; 1.
PBIL [Tree]
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
ProtoNet Q9HYD5.
Genome annotation databases
GeneID 879925; -.
GenomeReviews AE004091_GR; PA3471.
KEGG pae:PA3471; -.
NMPDR fig|208964.1.peg.3471; -.
Phylogenomic databases
HOGENOM Q9HYD5; -.
Genome annotation databases
CMR Q9HYD5; PA3471.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   564  564     NAD-dependent malic enzyme. PRO_0000160224
ACT_SITE   102   102        Proton donor (By similarity). 
ACT_SITE   173   173        Proton acceptor (By similarity). 
METAL   244   244        Divalent metal cation (By similarity). 
METAL   245   245        Divalent metal cation (By similarity). 
METAL   268   268        Divalent metal cation (By similarity). 
BINDING   155   155        NAD (By similarity). 
BINDING   268   268        NAD (By similarity). 
BINDING   417   417        NAD (By similarity). 
SITE   268   268  1     Important for activity (By similarity). 
Sequence information
Length: 564 AA [This is the length of the unprocessed precursor] Molecular weight: 62420 Da [This is the MW of the unprocessed precursor] CRC64: C11F0057882BA7AE [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTETAKRPLY VPHAGPSLLE MPLLNKGSAF STQERIDFNL QGLLPHNIET IEEQTERAYS 

        70         80         90        100        110        120 
QYNLCNTDLD RHIFLRSIQD NNETLFFRLL EEHLEEMMPI IYTPTVGQAC QEFSKIYRTH 

       130        140        150        160        170        180 
RGLFISYPDR ERIDDILRSA TKNNVKIVVV TDSERILGLG DQGIGGMGIP IGKLSLYTAC 

       190        200        210        220        230        240 
GGISPAYTLP VVLDVGTNNP DLLNDPMYMG WRHERVSGAQ YEEFVDLFIQ AIKRRWPNVL 

       250        260        270        280        290        300 
LQFEDFAQTN AMPLLERYKD ELCCFNDDIQ GTAAVAVGTL LAACKAKGEK LSEQTVTFVG 

       310        320        330        340        350        360 
AGSAGCGIAE QIIAAMQLEG LDEAQARRRI FMVDRWGLLT DDMSNLLDFQ HRLAQKRADL 

       370        380        390        400        410        420 
GAWGGQQGDD LALLEVIRNA RPTVLIGVSG QRGLFSEEVI RELHSHCKQP LVMPLSNPTS 

       430        440        450        460        470        480 
RVEATPQEIL NWTDGQALVA TGSPFQPVQV GDKRIPIAQC NNAYIFPGIG LGVIAARANR 

       490        500        510        520        530        540 
VTEGMLMAAA NALANCSPIV TQGEGAVLPA LGDIREVSKR IAVAVAKQAQ AEGKALHTSD 

       550        560 
EVLNDAIEAN FWFPRYRAYR RTSF
Q9HYD5 in FASTA format

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