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UniProtKB/Swiss-Prot entry Q9EVG6

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU3_BUCUA
Primary accession number Q9EVG6
Secondary accession numbers None
Integrated into Swiss-Prot on October 19, 2002
Sequence was last modified on March 1, 2001 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 40)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase
Synonyms EC 1.1.1.85
Beta-IPM dehydrogenase
IMDH
3-IPM-DH
Gene name
Name: leuB
From
Buchnera aphidicola subsp. Uroleucon aeneum [TaxID: 118112] 
Encoded on Plasmid pLeu (pBAp1).
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Buchnera.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1128/JB.183.2.785-790.2001; PubMed=11133977 [NCBI, ExPASy, EBI, Israel, Japan]
Wernegreen J.J., Moran N.A.;
"Vertical transmission of biosynthetic plasmids in aphid endosymbionts (Buchnera).";
J. Bacteriol. 183:785-790(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF197455; AAG31399.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
3D structure databases
HSSP P37412; 1CNZ. [HSSP ENTRY / PDB]
ModBase Q9EVG6.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145; Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009098; Biological process: leucine biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
HAMAP MF_01033; -; 1.
PBIL [Tree]
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
ProtoNet Q9EVG6.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase; Plasmid.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   363  363     3-isopropylmalate dehydrogenase. PRO_0000083663
NP_BIND   78    91  14     NAD (By similarity). 
NP_BIND   285   297  13     NAD (By similarity). 
METAL   227   227        Magnesium or manganese (By similarity). 
METAL   251   251        Magnesium or manganese (By similarity). 
METAL   255   255        Magnesium or manganese (By similarity). 
BINDING   99    99        Substrate (By similarity). 
BINDING   109   109        Substrate (By similarity). 
BINDING   138   138        Substrate (By similarity). 
BINDING   227   227        Substrate (By similarity). 
SITE   145   145  1     Important for catalysis (By similarity). 
SITE   195   195  1     Important for catalysis (By similarity). 
Sequence information
Length: 363 AA [This is the length of the unprocessed precursor] Molecular weight: 40524 Da [This is the MW of the unprocessed precursor] CRC64: 15E972DAABFE6996 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTRKYRIAVL PGDGIGPEVM REAYKILNIL KDDFSLPLEI KEFNIGGAAI DQEGVALPKK 

        70         80         90        100        110        120 
TLLGCENSDA ILFGSVGGKK WDNLPINQRP ERASLLPLRK HFNLFANLRP AQLYSELKNL 

       130        140        150        160        170        180 
SPLRSNIIKN GFNILCIREL TGGIYFGKPT GRLKKNNIEY AFDTEIYYNY EINRIAHLAF 

       190        200        210        220        230        240 
QLSQTRNYKV CSIDKSNVLN SSILWREIVK KVSKHYPDVH LSHLYIDNAT MQIIKDPNQF 

       250        260        270        280        290        300 
DVLLCSNLFG DIISDECAMI TGSIGMLPSA SLNEKKFGLY EPAGGSAPDI EGKNIANPIA 

       310        320        330        340        350        360 
QILSLSMLVR YSMNLKKIAD KIDQSVISVL KKGYRTADIS NNNDYLKTNE MGDVIASALI 


SGE
Q9EVG6 in FASTA format

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