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UniProtKB/Swiss-Prot entry Q9EVG3

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU3_BUCML
Primary accession number Q9EVG3
Secondary accession numbers None
Integrated into Swiss-Prot on October 19, 2002
Sequence was last modified on March 1, 2001 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 40)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase
Synonyms EC 1.1.1.85
Beta-IPM dehydrogenase
IMDH
3-IPM-DH
Gene name
Name: leuB
From
Buchnera aphidicola subsp. Macrosiphoniella ludovicianae [TaxID: 118105] 
Encoded on Plasmid pLeu (pBAp1).
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Buchnera.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1128/JB.183.2.785-790.2001; PubMed=11133977 [NCBI, ExPASy, EBI, Israel, Japan]
Wernegreen J.J., Moran N.A.;
"Vertical transmission of biosynthetic plasmids in aphid endosymbionts (Buchnera).";
J. Bacteriol. 183:785-790(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF197456; AAG31402.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
3D structure databases
HSSP P37412; 1CNZ. [HSSP ENTRY / PDB]
ModBase Q9EVG3.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145; Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009098; Biological process: leucine biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
HAMAP MF_01033; -; 1.
PBIL [Tree]
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
ProtoNet Q9EVG3.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase; Plasmid.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   365  365     3-isopropylmalate dehydrogenase. PRO_0000083658
NP_BIND   78    91  14     NAD (By similarity). 
NP_BIND   286   298  13     NAD (By similarity). 
METAL   228   228        Magnesium or manganese (By similarity). 
METAL   252   252        Magnesium or manganese (By similarity). 
METAL   256   256        Magnesium or manganese (By similarity). 
BINDING   99    99        Substrate (By similarity). 
BINDING   109   109        Substrate (By similarity). 
BINDING   139   139        Substrate (By similarity). 
BINDING   228   228        Substrate (By similarity). 
SITE   146   146  1     Important for catalysis (By similarity). 
SITE   196   196  1     Important for catalysis (By similarity). 
Sequence information
Length: 365 AA [This is the length of the unprocessed precursor] Molecular weight: 40966 Da [This is the MW of the unprocessed precursor] CRC64: 213AEB0F71DF960C [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MKKQYRIAVL PGDGIGPEVM KEAYKILKIL QNYFSLHIEI KEFNIGGIAL DKEGVALPKD 

        70         80         90        100        110        120 
TLLGCENSDA ILFGSVGGKK WNNLPIEKRP ERASLLPLRK HFNLFYNLRP AKLYSELRFL 

       130        140        150        160        170        180 
SPLRSKTXIK NGFDILCIRE LTGGIYFGEP RGRLKKNNIE YAFDTEVYYD YEINRIAHVA 

       190        200        210        220        230        240 
FQLARTRKNK VCSIDKANVL NSSLLWREIV TKVSKNYPDV HLSHLYIDNA TMQIIQNPNQ 

       250        260        270        280        290        300 
FDILLCSNLF GDILSDECAI ITGSIGMLPS ASLNEKNFGL YEPAGGSAPD IAGKNIANPI 

       310        320        330        340        350        360 
AQILSLSMLV RYGMNLKKIA NKIDQSVISV LKKGYRTFDI SDDNNXFLKT NEMGDVIADA 


LINGE
Q9EVG3 in FASTA format

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