[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.; "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."; Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: (R)-2,3-dihydroxy-3-methylbutanoate + NADP⁺ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
CATALYTIC ACTIVITY: (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP⁺ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4 .
PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4 .
SIMILARITY: Belongs to the ketol-acid reductoisomerase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AP006627; BAD65178.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_176139.1; -.

3D structure databases

ModBase

Q5WEN2.

Enzyme and pathway databases

BioCyc

BCLA66692:ABC2643-MON; -.

Ontologies

GO:0005488;	Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0004455;	Molecular function: ketol-acid reductoisomerase activity (inferred from electronic annotation from HAMAP).
GO:0009097;	Biological process: isoleucine biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0009099;	Biological process: valine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00435; -; 1.
PBIL [Tree]

InterPro

IPR013023; AcH_isomrdctse.
IPR000506; AcH_isomrdctse_C.
IPR013116; IlvN.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR21371; AcH_isomrdctse; 1.

Pfam

PF01450; IlvC; 1.
PF07991; IlvN; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00465; ilvC; 1.

ProtoNet

Q5WEN2.

Genome annotation databases

GeneID

3201311; -.

GenomeReviews

AP006627_GR; ABC2643.

KEGG

bcl:ABC2643; -.

NMPDR

fig|66692.3.peg.2940; -.

Phylogenomic databases

HOGENOM

Q5WEN2; -.

Genome annotation databases

CMR

Q5WEN2; ABC2643.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 341`	`341`	`Ketol-acid reductoisomerase.`	`PRO_0000226157`
`ACT_SITE`	`107 107`		`Potential.`

Sequence information

Length: 341 AA [This is the length of the unprocessed precursor]

Molecular weight: 37262 Da [This is the MW of the unprocessed precursor]

CRC64: 1B80C1C485792761 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAKVYYNGDV NEQVLEGKTV AIVGYGSQGH AHAQNLRESG VDVIIGLRPG KSWDKATEDG 

        70         80         90        100        110        120 
FSVYSVREAS AKADIIMILS PDEHQPAIYK ESIEPELTAG KALVFAHGFN IHFNQIVPPE 

       130        140        150        160        170        180 
HVDVFLAAPK GPGHLVRRTY TEGAGVPALI AVYQDATGQA KDIALAYAKQ IGAARAGVLE 

       190        200        210        220        230        240 
TTFKEETETD LFGEQAVLCG GTSALVKAGF ETLVEAGYQP EIAYFECLHE LKLIVDLMYE 

       250        260        270        280        290        300 
GGLEYMRYSI SDTAQWGDFQ AGPRIVTDET KAAMKEILSD IQKGKFAKGW ILENKLNRPE 

       310        320        330        340 
YNAINEAEKN HPLEVVGREL REMMPFVKAS KSKGVVANAK N

Q5WEN2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools