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UniProtKB/Swiss-Prot entry P87256

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU3A_ASPNG
Primary accession number P87256
Secondary accession numbers None
Integrated into Swiss-Prot on May 30, 2000
Sequence was last modified on July 1, 1997 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 53)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase A
Synonyms 3-IPM-DH A
IMDH A
EC 1.1.1.85
Beta-IPM dehydrogenase A
Gene name
Name: leu2A
From
Aspergillus niger [TaxID: 5061] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; Aspergillus.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=A733;
DOI=10.1007/s002940050137; PubMed=8781173 [NCBI, ExPASy, EBI, Israel, Japan]
Williams B.A., Sillaots S., Tsang A., Storms R.;
"Isolation by genetic complementation of two differentially expressed genes for beta-isopropylmalate dehydrogenase from Aspergillus niger.";
Curr. Genet. 30:305-311(1996).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
S83228; AAB50827.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U51130; AAD10278.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S72209; S72209.
3D structure databases
HSSP P12010; 2AYQ. [HSSP ENTRY / PDB]
ModBase P87256.
Ontologies
GO
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145; Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS P87256.
ProtoNet P87256.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   363  363     3-isopropylmalate dehydrogenase A. PRO_0000083599
NP_BIND   78    89  12     NAD (By similarity). 
NP_BIND   287   299  13     NAD (By similarity). 
METAL   222   222        Magnesium or manganese (By similarity). 
METAL   247   247        Magnesium or manganese (By similarity). 
METAL   251   251        Magnesium or manganese (By similarity). 
BINDING   96    96        Substrate (By similarity). 
BINDING   106   106        Substrate (By similarity). 
BINDING   135   135        Substrate (By similarity). 
BINDING   222   222        Substrate (By similarity). 
SITE   142   142  1     Important for catalysis (By similarity). 
SITE   189   189  1     Important for catalysis (By similarity). 
Sequence information
Length: 363 AA [This is the length of the unprocessed precursor] Molecular weight: 38670 Da [This is the MW of the unprocessed precursor] CRC64: 20AC729774C646B7 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MPAYNIVVFA GDHWGPEVTA EAIKVLRVIE KSRDDITLNL QDHLLGGASI DATANPLTDE 

        70         80         90        100        110        120 
ALAAAKNADA VLLGAIGGPK WGTGAVRPEQ GLLNVRKEMG TFGNLRPCNF AAPSLVEHSP 

       130        140        150        160        170        180 
LKASVCEGVD FNIIRELTGG IYFGDRKKMT AAATMDTEPY SRAEIERITP RAHLALQHNP 

       190        200        210        220        230        240 
PLPVWSLDKA NVLATSRLWR KTVTEIMAKE FPQLKIEHQL IDSAAMIMVK NPRQLNGIIV 

       250        260        270        280        290        300 
TSNLFGDIIS DEASVIPGSL GLLPSASLSG IPDGKGRVNG IYEPIHGSAP DIAGKGIVNP 

       310        320        330        340        350        360 
VAAILSVAMM MQYSFGRFDE ARAIEAAVRN VLESGVRTGD IGGKATTSEV GDAVAAELEK 


LLK
P87256 in FASTA format

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