FUNCTION: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate + NAD⁺ = 4-methyl-2-oxopentanoate + CO₂ + NADH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF000121; AAB58312.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P12010; 2AYQ. [HSSP ENTRY / PDB]

ModBase

P87186.

Ontologies

GO:0000287;	Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145;	Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.718.10; IDH_IMDH; 1.

PANTHER

PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.

Pfam

PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00169; leuB; 1.

PROSITE

PS00470; IDH_IMDH; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 373`	`373`	`3-isopropylmalate dehydrogenase.`	`PRO_0000083601`
`NP_BIND`	`82 93`	`12`	`NAD (By similarity).`
`NP_BIND`	`295 306`	`12`	`NAD (By similarity).`
`METAL`	`231 231`		`Magnesium or manganese (By similarity).`
`METAL`	`256 256`		`Magnesium or manganese (By similarity).`
`METAL`	`260 260`		`Magnesium or manganese (By similarity).`
`BINDING`	`100 100`		`Substrate (By similarity).`
`BINDING`	`110 110`		`Substrate (By similarity).`
`BINDING`	`139 139`		`Substrate (By similarity).`
`BINDING`	`231 231`		`Substrate (By similarity).`
`SITE`	`146 146`	`1`	`Important for catalysis (By similarity).`
`SITE`	`198 198`	`1`	`Important for catalysis (By similarity).`

Sequence information

Length: 373 AA [This is the length of the unprocessed precursor]

Molecular weight: 40193 Da [This is the MW of the unprocessed precursor]

CRC64: 4B0DCC23C6F52EAE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSVKTQTITV LPGDHVGTEI VNEPTKVLKA IEAPTPYQKI QFDFKHHLIG GAAIDATGVP 

        70         80         90        100        110        120 
LPDDALESAK SSDAVLLGAV GGPKWGTGTV RPEQGLLKIR KELNLYANIR PCNFASDSLL 

       130        140        150        160        170        180 
ELSPLKAEVV KGTNLIIVRE LVGGIYFGER QEQEESEDHQ TAWYRPKYTV DEVTRITRMA 

       190        200        210        220        230        240 
AFMALQHNPP LPIWSLDKAN VLASSRLWRK TVDKVISEEF PALSVQHQLI DSAAMILIQN 

       250        260        270        280        290        300 
PTKLSGIIIT SNMFGDIISD EASVIPGSLG LLPSASLASL PDTNTAFGLY EPCHGSAPDL 

       310        320        330        340        350        360 
PANKVNPIAT ILSAASMLRL ALDCVKETEA LEEAVKQVLD SGIRTADLRG TSSTTEVGDA 

       370 
IAETVTKILK QNT

P87186 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools