[1]	PROTEIN SEQUENCE. STRAIN=Nf 66; Omura M., Furushima-Shimogawara R., Izumiyama S., Endo T.; "Comparative study of protein profiles on pathogenic and nonpathogenic Naegleria species by 2D-PAGE."; Submitted (DEC-2003) to UniProtKB.

Comments

CATALYTIC ACTIVITY: Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
CATALYTIC ACTIVITY: Oxalosuccinate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
COFACTOR: Manganese or magnesium (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: 6.8, its MW is: 32.3 kDa.
SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

3D structure databases

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0000287;	Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145;	Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006099;	Biological process: tricarboxylic acid cycle (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

PTHR11822; IDH_NADP_euk; 1.

PS00470; IDH_IMDH; PARTIAL.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Direct protein sequencing; Magnesium; Manganese; NADP; Oxidoreductase; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 >20`	`>20`	`Isocitrate dehydrogenase [NADP].`	`PRO_0000083582`
`NON_TER`	`20 20`

Sequence information

Length: 20 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 2215 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 4A8244E9BA01D677 [This is a checksum on the sequence]

        10         20 
AXFQKIKVAN PVVELDGDEM

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools