PROTEIN SEQUENCE.
STRAIN=ATCC 11996 / DSM 50244 / JCM 5832 / NCIB 8955 / NCTC 10698;
PubMed=8944761 [NCBI, ExPASy, EBI, Israel, Japan]
Oppermann U.C.T., Maser E.;
"Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the Gram-negative bacterium Comamonas testosteroni.";
Eur. J. Biochem. 241:744-749(1996).

Comments

FUNCTION: Along with the 3 alpha-hydroxysteroid dehydrogenase and 3-oxo-reductase activities towards a variety of cis or trans fused A/B ring steroids, it also reduces several xenobiotic carbonyl compounds, including a metyrapone-based class of insecticides, to the respective alcohol metabolites.
CATALYTIC ACTIVITY: Androsterone + NAD(P)⁺ = 5-alpha-androstane-3,17-dione + NAD(P)H.
SUBCELLULAR LOCATION: Cytoplasm.
INDUCTION: By steroids.
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

3D structure databases

HSSP

Q9ZFY9; 1FK8. [HSSP ENTRY / PDB]

ModBase

P80702.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0047042;	Molecular function: 3-alpha-hydroxysteroid dehydrogenase (B-specific) activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002198; DHase_sc/Rdtase_SDR.
Graphical view of domain structure.

PROSITE

PS00061; ADH_SHORT; PARTIAL.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 >30`	`>30`	`3-alpha-hydroxysteroid dehydrogenase.`	`PRO_0000054656`
`REGION`	`10 >30`	`>21`	`Involved in cofactor binding (By similarity).`
`BINDING`	`29 29`		`Substrate (By similarity).`
`NON_TER`	`30 30`

Sequence information

Length: 30 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 2829 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 065E9CF03F1C5A29 [This is a checksum on the sequence]

        10         20         30 
DIIVISGAAT GIGAATCKVL EAAGSQIVSI

P80702 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools