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UniProtKB/Swiss-Prot entry P78827


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ILV5_SCHPO
Primary accession number P78827
Secondary accession number O42619
Integrated into Swiss-Prot on May 30, 2000
Sequence was last modified on January 11, 2001 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 65)
Name and origin of the protein
Protein name Probable ketol-acid reductoisomerase, mitochondrial [Precursor]
Synonyms EC 1.1.1.86
Acetohydroxy-acid reductoisomerase
Alpha-keto-beta-hydroxylacil reductoisomerase
Gene name
Name: ilv5
ORFNames: SPBC56F2.12
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=PR745;
DOI=10.1093/dnares/4.6.363; PubMed=9501991 [NCBI, ExPASy, EBI, Israel, Japan]
Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.;
"Identification of open reading frames in Schizosaccharomyces pombe cDNAs.";
DNA Res. 4:363-369(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
[3]
NUCLEOTIDE SEQUENCE [MRNA] OF 262-404.
Kawamukai M.;
"S.pombe ILV5 homolog.";
Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-261, AND MASS SPECTROMETRY.
DOI=10.1021/pr7006335; PubMed=18257517 [NCBI, ExPASy, EBI, Israel, Japan]
Wilson-Grady J.T., Villen J., Gygi S.P.;
"Phosphoproteome analysis of fission yeast.";
J. Proteome Res. 7:1088-1097(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D89175; BAA13837.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CU329671; CAA18891.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB009603; BAA24000.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T40532; T40532.
RefSeq NP_001018845.2; -.
3D structure databases
HSSP Q9HVA2; 1NP3. [HSSP ENTRY / PDB]
ModBase P78827.
Enzyme and pathway databases
BioCyc SPOM-XXX-01:SPOM-XXX-01-004692-MON; -.
Organism-specific databases
GeneDB_Spombe SPBC56F2.12; -.
Gene expression databases
ArrayExpress P78827; -.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from GeneDB_SPombe).
QuickGo view.
Family and domain databases
InterPro IPR013023; AcH_isomrdctse.
IPR000506; AcH_isomrdctse_C.
IPR013328; DHase_multihelical.
IPR013116; IlvN.
IPR016207; KetolA_reductoisomerase_fun.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
G3DSA:1.10.1040.10; Opine_DH; 1.
PANTHER PTHR21371; AcH_isomrdctse; 1.
Pfam PF01450; IlvC; 1.
PF07991; IlvN; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000119; Ilv5_fungal; 1.
TIGRFAMs TIGR00465; ilvC; 1.
BLOCKS P78827.
ProtoNet P78827.
Genome annotation databases
GeneID 3361354; -.
KEGG spo:SPBC56F2.12; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Mitochondrion; NADP; Oxidoreductase; Phosphoprotein; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1     ?        Mitochondrion (Potential). 
CHAIN   ?   404        Probable ketol-acid reductoisomerase, mitochondrial. PRO_0000015633
ACT_SITE   178   178        Potential. 
MOD_RES   261   261        Phosphoserine. 
CONFLICT   38    38        S -> R (in Ref. 1; BAA13837). 
CONFLICT   92    92        Y -> S (in Ref. 1; BAA13837). 
CONFLICT   275   275        N -> P (in Ref. 1; BAA13837). 
CONFLICT   297   297        N -> P (in Ref. 1; BAA13837). 
CONFLICT   394   394        V -> G (in Ref. 1; BAA13837). 
Sequence information
Length: 404 AA [This is the length of the unprocessed precursor] Molecular weight: 45189 Da [This is the MW of the unprocessed precursor] CRC64: 9AB3674C71AD6FEB [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSFRNSSRMA MKALRTMGSR RLATRSMSVM ARTIAAPSMR FAPRMTAPLM QTRGMRVMDF 

        70         80         90        100        110        120 
AGTKENVWER SDWPREKLVD YFKNDTLAII GYGSQGHGQG LNARDQGLNV IVGVRKDGAS 

       130        140        150        160        170        180 
WKQAIEDGWV PGKTLFPVEE AIKKGSIIMN LLSDAAQTET WPKIAPLITK GKTLYFSHGF 

       190        200        210        220        230        240 
SVIFKDQTKI HPPKDVDVIL VAPKGSGRTV RTLFKEGRGI NSSFAVYQDV TGKAQEKAIG 

       250        260        270        280        290        300 
LAVAVGSGFI YQTTFKKEVI SDLVGERGCL MGGINGLFLA QYQVLRERGH SPAEAFNETV 

       310        320        330        340        350        360 
EEATQSLYPL IGKYGLDYMF AACSTTARRG AIDWTPRFLE ANKKVLNELY DNVENGNEAK 

       370        380        390        400 
RSLEYNSAPN YRELYDKELE EIRNLEIWKA GEVVRSLRPE HNKH 

P78827 in FASTA format

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