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UniProtKB/Swiss-Prot entry P71753


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SIR_MYCTU
Primary accession number P71753
Secondary accession number Q7D781
Integrated into Swiss-Prot on September 27, 2005
Sequence was last modified on September 27, 2005 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 56)
Name and origin of the protein
Protein name Sulfite reductase [ferredoxin]
Synonym EC 1.8.7.1
Gene name
Name: sir
Synonyms: nirA
OrderedLocusNames: Rv2391, MT2461
From
Mycobacterium tuberculosis [TaxID: 1773] [HAMAP proteome]
Taxonomy Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Corynebacterineae; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 25618 / H37Rv;
DOI=10.1038/31159; PubMed=9634230 [NCBI, ExPASy, EBI, Israel, Japan]
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.;
"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.";
Nature 393:537-544(1998).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=CDC 1551 / Oshkosh;
DOI=10.1128/JB.184.19.5479-5490.2002; PubMed=12218036 [NCBI, ExPASy, EBI, Israel, Japan]
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains.";
J. Bacteriol. 184:5479-5490(2002).
[3]
X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, AND MUTAGENESIS OF TYR-69 AND CYS-161.
STRAIN=ATCC 25618 / H37Rv;
DOI=10.1074/jbc.M502560200; PubMed=15917234 [NCBI, ExPASy, EBI, Israel, Japan]
Schnell R., Sandalova T., Hellman U., Lindqvist Y., Schneider G.;
"Siroheme- and [Fe4-S4]-dependent NirA from Mycobacterium tuberculosis is a sulfite reductase with a covalent Cys-Tyr bond in the active site.";
J. Biol. Chem. 280:27319-27328(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
BX842579; CAB03734.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE000516; AAK46756.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B70682; B70682.
RefSeq NP_216907.1; -.
NP_336942.1; -.
3D structure databases
PDB
1ZJ8; X-ray; 2.80 A; A/B=1-555.[ExPASy / RCSB / EBI]
1ZJ9; X-ray; 2.90 A; A/B=1-555.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1ZJ8; -.
1ZJ9; -.
ModBase P71753.
Organism-specific databases
TubercuList Rv2391; -.
Ontologies
GO
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0050311; Molecular function: sulfite reductase (ferredoxin) activity (inferred from electronic annotation from EC).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR006066; Nir_Si_BS.
IPR006067; Nir_Sir_4Fe4S.
IPR005117; NiRdtase/SiRdtase_haem-b_fer.
Graphical view of domain structure.
Pfam PF01077; NIR_SIR; 2.
PF03460; NIR_SIR_ferr; 2.
Pfam graphical view of domain structure.
PRINTS PR00397; SIROHAEM.
PROSITE PS00365; NIR_SIR; 1.
BLOCKS P71753.
ProtoNet P71753.
Genome annotation databases
GeneID 885472; -.
925885; -.
GenomeReviews AE000516_GR; MT2461.
AL123456_GR; Rv2391.
KEGG mtc:MT2461; -.
mtu:Rv2391; -.
TIGR MT2461; -.
Phylogenomic databases
HOGENOM P71753; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; 4Fe-4S; Complete proteome; Heme; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Thioether bond.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   555  555     Sulfite reductase [ferredoxin]. PRO_0000199952
METAL   417   417        Iron-sulfur (4Fe-4S). 
METAL   423   423        Iron-sulfur (4Fe-4S). 
METAL   463   463        Iron-sulfur (4Fe-4S). 
METAL   467   467        Iron (siroheme axial ligand). 
METAL   467   467        Iron-sulfur (4Fe-4S). 
CROSSLNK   69   161        3'-(S-cysteinyl)-tyrosine (Tyr-Cys). 
MUTAGEN   69    69        Y->A,F: Strong decrease in activity. 
MUTAGEN   161   161        C->A,S: Strong decrease in activity. 
HELIX   15    17  3      
HELIX   25    29  5      
HELIX   30    32  3      
HELIX   35    37  3      
HELIX   38    44  7      
HELIX   46    49  4      
HELIX   51    53  3      
HELIX   56    60  5      
HELIX   62    66  5      
STRAND   68    72  5      
HELIX   78    80  3      
HELIX   83    85  3      
HELIX   86    89  4      
STRAND   90    99  10      
HELIX   101   103  3      
HELIX   107   120  14      
STRAND   124   127  4      
STRAND   133   138  6      
HELIX   140   142  3      
HELIX   143   151  9      
TURN   152   154  3      
STRAND   160   170  11      
TURN   172   176  5      
HELIX   185   195  11      
HELIX   199   201  3      
STRAND   208   216  9      
HELIX   220   222  3      
STRAND   223   232  10      
TURN   233   235  3      
STRAND   236   243  8      
STRAND   248   250  3      
STRAND   255   261  7      
HELIX   263   265  3      
HELIX   266   280  15      
HELIX   286   288  3      
HELIX   291   298  8      
HELIX   300   310  11      
STRAND   333   337  5      
STRAND   340   347  8      
STRAND   352   354  3      
HELIX   355   368  14      
STRAND   373   375  3      
STRAND   381   386  6      
HELIX   388   400  13      
STRAND   404   406  3      
HELIX   409   413  5      
STRAND   414   416  3      
HELIX   419   421  3      
HELIX   431   444  14      
HELIX   446   449  4      
STRAND   458   463  6      
HELIX   470   472  3      
STRAND   473   487  15      
STRAND   489   498  10      
TURN   518   520  3      
HELIX   521   534  14      
HELIX   542   548  7      
HELIX   551   554  4      
Sequence information
Length: 555 AA [This is the length of the unprocessed precursor] Molecular weight: 62112 Da [This is the MW of the unprocessed precursor] CRC64: 68925CA37C131BC3 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTTARPAKAR NEGQWALGHR EPLNANEELK KAGNPLDVRE RIENIYAKQG FDSIDKTDLR 

        70         80         90        100        110        120 
GRFRWWGLYT QREQGYDGTW TGDDNIDKLE AKYFMMRVRC DGGALSAAAL RTLGQISTEF 

       130        140        150        160        170        180 
ARDTADISDR QNVQYHWIEV ENVPEIWRRL DDVGLQTTEA CGDCPRVVLG SPLAGESLDE 

       190        200        210        220        230        240 
VLDPTWAIEE IVRRYIGKPD FADLPRKYKT AISGLQDVAH EINDVAFIGV NHPEHGPGLD 

       250        260        270        280        290        300 
LWVGGGLSTN PMLAQRVGAW VPLGEVPEVW AAVTSVFRDY GYRRLRAKAR LKFLIKDWGI 

       310        320        330        340        350        360 
AKFREVLETE YLKRPLIDGP APEPVKHPID HVGVQRLKNG LNAVGVAPIA GRVSGTILTA 

       370        380        390        400        410        420 
VADLMARAGS DRIRFTPYQK LVILDIPDAL LDDLIAGLDA LGLQSRPSHW RRNLMACSGI 

       430        440        450        460        470        480 
EFCKLSFAET RVRAQHLVPE LERRLEDINS QLDVPITVNI NGCPNSCARI QIADIGFKGQ 

       490        500        510        520        530        540 
MIDDGHGGSV EGFQVHLGGH LGLDAGFGRK LRQHKVTSDE LGDYIDRVVR NFVKHRSEGE 

       550 
RFAQWVIRAE EDDLR 

P71753 in FASTA format

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