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UniProtKB/Swiss-Prot entry P69167

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name HSD_MYCTU
Primary accession number P69167
Secondary accession number Q10855
Integrated into Swiss-Prot on February 15, 2005
Sequence was last modified on February 15, 2005 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 28)
Name and origin of the protein
Protein name 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase
Synonym EC 1.1.1.53
Gene name
Name: fabG3
OrderedLocusNames: Rv2002, MT2058
ORFNames: MTCY39.16c
From
Mycobacterium tuberculosis [TaxID: 1773] [HAMAP proteome]
Taxonomy Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Corynebacterineae; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 25618 / H37Rv;
DOI=10.1038/31159; PubMed=9634230 [NCBI, ExPASy, EBI, Israel, Japan]
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.;
"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.";
Nature 393:537-544(1998).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=CDC 1551 / Oshkosh;
DOI=10.1128/JB.184.19.5479-5490.2002; PubMed=12218036 [NCBI, ExPASy, EBI, Israel, Japan]
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains.";
J. Bacteriol. 184:5479-5490(2002).
[3]
 X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF MUTANTS THR-6; MET-47 AND LYS-69.
STRAIN=ATCC 25618 / H37Rv;
DOI=10.1073/pnas.0137017100; PubMed=12524453 [NCBI, ExPASy, EBI, Israel, Japan]
Yang J.K., Park M.S., Waldo G.S., Suh S.W.;
"Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis.";
Proc. Natl. Acad. Sci. U.S.A. 100:455-460(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
BX842578; CAA98414.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE000516; AAK46335.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR H70758; H70758.
RefSeq NP_216518.1; -.
NP_336521.1; -.
3D structure databases
PDB
1NFF; X-ray; 1.80 A; A/B=1-260.[ExPASy / RCSB / EBI]
1NFQ; X-ray; 2.40 A; A/B/C/D=1-260.[ExPASy / RCSB / EBI]
1NFR; X-ray; 2.10 A; A/B/C/D=1-260.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1NFF; -.
1NFQ; -.
1NFR; -.
ModBase P69167.
Organism-specific databases
TubercuList Rv2002; -.
Ontologies
GO
GO:0047044; Molecular function: 3-alpha(or 20-beta)-hydroxysteroid dehydrogenase activity (inferred from electronic annotation from EC).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0008202; Biological process: steroid metabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR19410; ADH_short_C2; 1.
Pfam PF00106; adh_short; 1.
Pfam graphical view of domain structure.
PRINTS PR00081; GDHRDH.
PR00080; SDRFAMILY.
PROSITE PS00061; ADH_SHORT; 1.
BLOCKS P69167.
ProtoNet P69167.
Genome annotation databases
GeneID 888857; -.
923379; -.
GenomeReviews AE000516_GR; MT2058.
AL123456_GR; Rv2002.
KEGG mtc:MT2058; -.
mtu:Rv2002; -.
TIGR MT2058; -.
Phylogenomic databases
HOGENOM P69167; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Lipid metabolism; NAD; Oxidoreductase; Steroid metabolism.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   260  260     3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase. PRO_0000054712
NP_BIND   11    35  25     NAD (By similarity). 
ACT_SITE   153   153        Proton acceptor. 
BINDING   140   140        Substrate. 
CONFLICT   174   174        S -> G (in Ref. 2; AAK46335). 
TURN   4     7  4      
STRAND   9    13  5      
TURN   14    16  3      
HELIX   18    29  12      
STRAND   33    39  7      
HELIX   41    50  10      
HELIX   52    54  3      
STRAND   55    59  5      
HELIX   65    79  15      
STRAND   84    87  4      
TURN   97    99  3      
HELIX   102   112  11      
HELIX   114   130  17      
STRAND   133   138  6      
HELIX   141   143  3      
HELIX   151   171  21      
HELIX   172   174  3      
STRAND   176   183  8      
HELIX   189   191  3      
STRAND   202   205  4      
HELIX   209   220  12      
HELIX   222   224  3      
STRAND   231   235  5      
HELIX   238   240  3      
Sequence information
Length: 260 AA [This is the length of the unprocessed precursor] Molecular weight: 27030 Da [This is the MW of the unprocessed precursor] CRC64: 0935A14ED36220B7 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSGRLIGKVA LVSGGARGMG ASHVRAMVAE GAKVVFGDIL DEEGKAVAAE LADAARYVHL 

        70         80         90        100        110        120 
DVTQPAQWTA AVDTAVTAFG GLHVLVNNAG ILNIGTIEDY ALTEWQRILD VNLTGVFLGI 

       130        140        150        160        170        180 
RAVVKPMKEA GRGSIINISS IEGLAGTVAC HGYTATKFAV RGLTKSTALE LGPSGIRVNS 

       190        200        210        220        230        240 
IHPGLVKTPM TDWVPEDIFQ TALGRAAEPV EVSNLVVYLA SDESSYSTGA EFVVDGGTVA 

       250        260 
GLAHNDFGAV EVSSQPEWVT
P69167 in FASTA format

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