[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION. STRAIN=ATCC 21998 / FERM P-2439; Anderson S., Lazarus R.A., Miller H.I., Stafford R.K.; "Metabolic pathway engineering to increase production of ascorbic acid intermediates."; Patent number US5032514, 16-JUL-1991.

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively (By similarity).
CATALYTIC ACTIVITY: Glycolate + NADP⁺ = glyoxylate + NADPH.
CATALYTIC ACTIVITY: D-glycerate + NAD(P)⁺ = hydroxypyruvate + NAD(P)H.
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

3D structure databases

HSSP

P36234; 1GDH. [HSSP ENTRY / PDB]

ModBase

P58000.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from HAMAP).
GO:0030267;	Molecular function: glyoxylate reductase (NADP) activity (inferred from electronic annotation from HAMAP).
GO:0016618;	Molecular function: hydroxypyruvate reductase activity (inferred from electronic annotation from HAMAP).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

HAMAP

MF_01667; -; 1.
PBIL [Tree]

InterPro

IPR006139; D-isomer_2_OHA_DHase.
IPR006140; D-isomer_2_OHA_DHase_NAD-bd.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF00389; 2-Hacid_dh; 1.
PF02826; 2-Hacid_dh_C; 1.
Pfam graphical view of domain structure.

PROSITE

PS00065; D_2_HYDROXYACID_DH_1; FALSE_NEG.
PS00670; D_2_HYDROXYACID_DH_2; FALSE_NEG.
PS00671; D_2_HYDROXYACID_DH_3; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; NAD; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 323`	`323`	`Glyoxylate/hydroxypyruvate reductase B.`	`PRO_0000076031`
`ACT_SITE`	`236 236`		`By similarity.`
`ACT_SITE`	`265 265`		`By similarity.`
`ACT_SITE`	`284 284`		`Proton donor (By similarity).`

Sequence information

Length: 323 AA [This is the length of the unprocessed precursor]

Molecular weight: 35336 Da [This is the MW of the unprocessed precursor]

CRC64: BD778BDFDDF53AD1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKPEVLLYKS LPDDLRARLD EHFTVTAING LSPETIAEHG GAGARRRHDR LQQHGGSSAA 

        70         80         90        100        110        120 
GENAKLRAAS TISVGYDNFD VEALNQRGIV LIDTPTVLTE TVADTMMALV LSSARRVVEV 

       130        140        150        160        170        180 
AERVKAGEWR RSIGPDWFGI DVHHKKMGIL GMGRIGLALA QRAHHGFGMP ILYNARKHHE 

       190        200        210        220        230        240 
EAESRFNAQY CDLDTLLRES DFLCISLPLT EQTHHMIGRE QLAKMKPSAI LINAGRGPVV 

       250        260        270        280        290        300 
DEQALIAALK DKTIHAAGLD VFEQEPLPVD SELLTLPNVV ALPHIGSATH ETRYGMARDA 

       310        320 
VDNLIAALAG KVEKNCVNPQ VLR

P58000 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools