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UniProtKB/Swiss-Prot entry P57106


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MDHC2_ARATH
Primary accession number P57106
Secondary accession number Q9LSX3
Integrated into Swiss-Prot on December 1, 2000
Sequence was last modified on December 1, 2000 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 65)
Name and origin of the protein
Protein name Malate dehydrogenase, cytoplasmic 2
Synonym EC 1.1.1.37
Gene name
OrderedLocusNames: At5g43330
ORFNames: MWF20.2
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.1.31; PubMed=10718197 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:31-63(2000).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB025638; BAA97412.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY072137; AAL59959.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY091220; AAM14159.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_199147.1; -.
UniGene At.27775
3D structure databases
HSSP P11708; 4MDH. [HSSP ENTRY / PDB]
ModBase P57106.
Organism-specific databases
TAIR At5g43330; -.
Gene expression databases
ArrayExpress P57106; -.
GermOnline AT5G43330; Arabidopsis thaliana.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006099; Biological process: tricarboxylic acid cycle (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001557; L-lactate/malate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR001252; Malate_DHase_AS.
IPR011274; Malate_DHase_NAD-dep_euk.
IPR010945; Malate_DHase_SF1.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR23382; MDH_SF1; 1.
Pfam PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000102; Lac_mal_DH; 1.
ProDom PD003052; Mal_dehydrog; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01759; MalateDH-SF1; 1.
TIGR01758; MDH_euk_cyt; 1.
PROSITE PS00068; MDH; 1.
BLOCKS P57106.
ProtoNet P57106.
Genome annotation databases
GeneID 834351; -.
GenomeReviews BA000015_GR; AT5G43330.
KEGG ath:AT5G43330; -.
NMPDR fig|3702.1.peg.26071; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; NAD; Oxidoreductase; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   332  332     Malate dehydrogenase, cytoplasmic 2. PRO_0000113413
NP_BIND   12    18  7     NAD (By similarity). 
NP_BIND   130   132  3     NAD (By similarity). 
ACT_SITE   188   188        Proton acceptor (By similarity). 
BINDING   93    93        Substrate (By similarity). 
BINDING   99    99        Substrate (By similarity). 
BINDING   106   106        NAD (By similarity). 
BINDING   113   113        NAD (By similarity). 
BINDING   132   132        Substrate (By similarity). 
BINDING   163   163        Substrate (By similarity). 
Sequence information
Length: 332 AA [This is the length of the unprocessed precursor] Molecular weight: 35675 Da [This is the MW of the unprocessed precursor] CRC64: 76BF976943179FA7 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAKEPVRVLV TGAAGQIGYA LVPMIARGIM LGADQPVILH MLDIPFAAEA LNGVKMELVD 

        70         80         90        100        110        120 
AAFPLLKGVV ATTDAVEACT GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KSQASALEKH 

       130        140        150        160        170        180 
AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNITCLTRLD HNRALGQVSE RLSVPVSDVK 

       190        200        210        220        230        240 
NVIIWGNHSS TQYPDVNHAT VKTSVGEKPV RELVKNDEWL NGEFISTVQQ RGAAIIKARK 

       250        260        270        280        290        300 
LSSALSAASS ACDHIRDWVV GTPEGTFVSM GVYSDGSYNV PAGLIYSFPV TCRNGEWTIV 

       310        320        330 
QGLPIDDASR KKMDLTAEEL KEEKDLAYSC LS 

P57106 in FASTA format

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