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UniProtKB/Swiss-Prot entry P40926

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MDHM_HUMAN
Primary accession number P40926
Secondary accession number O43682
Integrated into Swiss-Prot on February 1, 1995
Sequence was last modified on May 30, 2000 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 92)
Name and origin of the protein
Protein name Malate dehydrogenase, mitochondrial [Precursor]
Synonym EC 1.1.1.37
Gene name
Name: MDH2
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
Hu G.;
"Human homolog of mouse and pig MDH mRNA.";
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Lung;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
 PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-56, AND MASS SPECTROMETRY.
DOI=10.1038/nbt1046; PubMed=15592455 [NCBI, ExPASy, EBI, Israel, Japan]
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.;
"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.";
Nat. Biotechnol. 23:94-101(2005).
[4]
 X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 20-338 IN COMPLEX WITH NAD AND SUBSTRATE, AND SUBUNIT.
Structural genomics consortium (SGC);
"Crystal structure of human malate dehydrogenase type 2.";
Submitted (MAR-2006) to the PDB data bank.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF047470; AAC03787.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC001917; AAH01917.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_005909.2; -.
UniGene Hs.520967
3D structure databases
PDB
2DFD; X-ray; 1.90 A; A/B/C/D=18-338.[ExPASy / RCSB / EBI]
PDBsum 2DFD; -.
ModBase P40926.
Protein-protein interaction databases
IntAct P40926; -.
PTM databases
PhosphoSite P40926; -.
Enzyme and pathway databases
Reactome REACT_1046; Pyruvate metabolism and TCA cycle.
REACT_1709; Metabolism of small molecules.
2D gel databases
DOSAC-COBS-2DPAGE P40926; -.
REPRODUCTION-2DPAGE IPI00291006; -.
P40926; -.
Organism-specific databases
H-InvDB HIX0006786; -.
HGNC HGNC:6971; MDH2.
GenAtlas MDH2.
MIM 154100; gene. [NCBI / EBI]
PharmGKB PA30716; -.
GeneCards P40926.
Gene expression databases
ArrayExpress P40926; -.
CleanEx HS_MDH2; -.
Ontologies
GO
GO:0005759; Cellular component: mitochondrial matrix (inferred from experiment from Reactome).
GO:0030060; Molecular function: L-malate dehydrogenase activity (inferred from direct assay from UniProtKB).
GO:0006108; Biological process: malate metabolic process (inferred from direct assay from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR001557; L-lactate/malate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR001252; Malate_DHase_AS.
IPR010097; Malate_DHase_NAD-dep_euk_g_bac.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11540:SF1; MDH_euk_g_bac; 1.
Pfam PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000102; Lac_mal_DH; 1.
TIGRFAMs TIGR01772; MDH_euk_gproteo; 1.
PROSITE PS00068; MDH; 1.
BLOCKS P40926.
ProtoNet P40926.
Genome annotation databases
Ensembl ENSG00000146701; Homo sapiens. [Contig view]
GeneID 4191; -.
KEGG hsa:4191; -.
Phylogenomic databases
HOGENOM P40926; -.
HOVERGEN P40926; -.
Other
DrugBank DB00157; NADH.
NextBio 16514; -.
SOURCE MDH2; Homo sapiens.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Acetylation; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Transit peptide; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    24  24     Mitochondrion (By similarity). 
CHAIN   25   338  314     Malate dehydrogenase, mitochondrial. PRO_0000018628
NP_BIND   31    37  7     NAD. 
NP_BIND   140   142  3     NAD. 
ACT_SITE   200   200        Proton acceptor (By similarity). 
BINDING   57    57        NAD. 
BINDING   104   104        Substrate. 
BINDING   110   110        Substrate. 
BINDING   117   117        NAD. 
BINDING   142   142        Substrate. 
BINDING   176   176        Substrate. 
BINDING   251   251        NAD. 
MOD_RES   56    56        Phosphotyrosine. 
MOD_RES   157   157        N6-acetyllysine (By similarity). 
MOD_RES   239   239        N6-acetyllysine (By similarity). 
MOD_RES   314   314        N6-acetyllysine (By similarity). 
STRAND   25    30  6      
TURN   31    33  3      
HELIX   37    45  9      
STRAND   50    60  11      
HELIX   61    69  9      
STRAND   71    74  4      
STRAND   76    82  7      
TURN   83    85  3      
HELIX   86    90  5      
STRAND   94    98  5      
HELIX   110   113  4      
HELIX   114   131  18      
STRAND   135   139  5      
HELIX   144   157  14      
STRAND   165   168  4      
HELIX   171   185  15      
HELIX   189   191  3      
STRAND   196   198  3      
HELIX   202   204  3      
STRAND   205   207  3      
HELIX   209   211  3      
HELIX   220   241  22      
HELIX   249   266  18      
STRAND   273   279  7      
STRAND   282   295  14      
STRAND   298   302  5      
HELIX   310   335  26      
Sequence information
Length: 338 AA [This is the length of the unprocessed precursor] Molecular weight: 35531 Da [This is the MW of the unprocessed precursor] CRC64: D375FA4FD10F63C1 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MLSALARPVS AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH 

        70         80         90        100        110        120 
TPGVAADLSH IETKAAVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI 

       130        140        150        160        170        180 
VATLTAACAQ HCPEAMICVI ANPVNSTIPI TAEVFKKHGV YNPNKIFGVT TLDIVRANTF 

       190        200        210        220        230        240 
VAELKGLDPA RVNVPVIGGH AGKTIIPLIS QCTPKVDFPQ DQLTALTGRI QEAGTEVVKA 

       250        260        270        280        290        300 
KAGAGSATLS MAYAGARFVF SLVDAMNGKE GVVECSFVKS QETECTYFST PLLLGKKGIE 

       310        320        330 
KNLGIGKVSS FEEKMISDAI PELKASIKKG EDFVKTLK
P40926 in FASTA format

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