NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=IAM 1030;
PubMed=1629157 [NCBI, ExPASy, EBI, Israel, Japan]
Nagao T., Mitamura T., Wang X.H., Negoro S., Yomo T., Urabe I., Okada H.;
"Cloning, nucleotide sequences, and enzymatic properties of glucose dehydrogenase isozymes from Bacillus megaterium IAM1030.";
J. Bacteriol. 174:5013-5020(1992).

Comments

CATALYTIC ACTIVITY: Beta-D-glucose + NAD(P)⁺ = D-glucono-1,5-lactone + NAD(P)H.
SUBUNIT: Homotetramer.
MISCELLANEOUS: Prefers NAD to NADP; 2M NaCl enhances its pH and thermostability.
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D10626; BAA01476.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

I40225; I40225.

3D structure databases

HSSP

P40288; 1GEE. [HSSP ENTRY / PDB]

SMR

P39485; 1-261.

ModBase

P39485.

Ontologies

GO:0047936;	Molecular function: glucose 1-dehydrogenase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

PROSITE

PS00061; ADH_SHORT; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 261`	`261`	`Glucose 1-dehydrogenase 4.`	`PRO_0000054612`
`NP_BIND`	`11 35`	`25`	`NAD (By similarity).`
`ACT_SITE`	`158 158`		`Proton acceptor (By similarity).`
`BINDING`	`145 145`		`Substrate (By similarity).`

Sequence information

Length: 261 AA [This is the length of the unprocessed precursor]

Molecular weight: 28157 Da [This is the MW of the unprocessed precursor]

CRC64: 6FBEC9397BCF417C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MYTDLKDKVV VITGGSTGLG RAMAVRFGQE EAKVVINYYN NEEEALDAKK EVEEAGGQAI 

        70         80         90        100        110        120 
IVQGDVTKEE DVVNLVQTAI KEFGTLDVMI NNAGVENPVP SHELSLDNWN KVIDTNLTGA 

       130        140        150        160        170        180 
FLGSREAIKY FVENDIKGNV INMSSVHEMI PWPLFVHYAA SKGGMKLMTE TLALEYAPKG 

       190        200        210        220        230        240 
IRVNNIGPGA MNTPINAEKF ADPVQRADVE SMIPMGYIGK PEEVAAVAAF LASSQASYVT 

       250        260 
GITLFADGGM TKYPSFQAGR G

P39485 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools