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- FUNCTION: Protein C packages viral RNA to form a viral nucleocapsid, and promotes virion budding (By similarity).
- FUNCTION: prM acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated (By similarity).
- FUNCTION: Envelope protein E binds cell surface receptor and is involved in membrane fusion between virion and target cell. Synthesized as an homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).
- FUNCTION: Non-structural protein 1 is slowly secreted from mammalian cells, but not from mosquito cells. Secreted form elicits protective immune response and plays an essential role in RNA replication. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome (By similarity).
- FUNCTION: Non-structural protein 2B is a required cofactor for the serine protease function of NS3 (By similarity).
- FUNCTION: Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction (By similarity).
- FUNCTION: Non-structural protein 4A plays a role in RNA replication. Enhances inhibition of cell antiviral response by non-structural protein 4B (By similarity).
- FUNCTION: Non-structural protein 4B prevent the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways (By similarity).
- FUNCTION: RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and assure the capping of genomes in the cytoplasm. May be involved in methylation of 5'RNA cap structure (By similarity).
- CATALYTIC ACTIVITY: Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
- SUBUNIT: prM and envelope protein E form heterodimers in the endoplasmic reticulum and Golgi. Envelope protein E forms homodimers. NS1 forms homodimers as well as homohexamers when secreted. NS1 may interact with NS4A. NS3 and NS2B form an heterodimer. NS3 interacts with unphosphorylated NS5 (By similarity).
- SUBCELLULAR LOCATION: Note=The virion is assembled in the endoplasmic reticulum lumen, transported by vesicles to the Golgi, then transported again to the cell membrane where it is released outside the cell.
- SUBCELLULAR LOCATION: Protein C: Virion (By similarity).
- SUBCELLULAR LOCATION: Peptide pr: Secreted (By similarity).
- SUBCELLULAR LOCATION: Small envelope protein M: Virion membrane; Single-pass type I membrane protein (By similarity).
- SUBCELLULAR LOCATION: Envelope protein E: Virion membrane; Single-pass type I membrane protein (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 1: Secreted. Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 2A-alpha: Endoplasmic reticulum membrane (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 2A: Endoplasmic reticulum membrane (By similarity).
- SUBCELLULAR LOCATION: Serine protease subunit NS2B: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
- SUBCELLULAR LOCATION: Serine protease subunit NS3: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 4A: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 4B: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=The C-terminal transmembrane domain of non-structural protein 4B is presumably reoriented after cleavage on the lumenal side (By similarity).
- SUBCELLULAR LOCATION: RNA-directed RNA polymerase NS5: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus (By similarity).
- DOMAIN: Transmembrane domains of the small envelope protein M and envelope protein E contains an endoplasmic reticulum retention signals (By similarity).
- PTM: Specific enzymatic cleavages in vivo yield mature proteins. The nascent protein C contains a C-terminal hydrophobic domain that act as a signal sequence for translocation of prM into the lumen of the ER. Mature protein C is cleaved at a site upstream of this hydrophobic domain by NS3. prM is cleaved in post-Golgi vesicles by a host furin, releasing the mature small envelope protein M, and peptide pr. Non-structural protein 2A-alpha, a C-terminally truncated form of non-structural protein 2A, results from partial cleavage by NS3 (By similarity).
- PTM: RNA-directed RNA polymerase NS5 is phosphorylated on serines residues. This phosphorylation may trigger NS5 nuclear localization (By similarity).
- PTM: Envelope protein E and non-structural protein 1 are N-glycosylated (By similarity).
- SIMILARITY: Contains 1 helicase ATP-binding domain.
- SIMILARITY: Contains 1 helicase C-terminal domain.
- SIMILARITY: Contains 1 peptidase S7 domain [view classification].
- SIMILARITY: Contains 1 RdRp catalytic domain.
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Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 3396 AA [This is the length of the unprocessed precursor] |
Molecular weight: 379564 Da [This is the MW of the unprocessed precursor] |
CRC64: C53E75F3E424367D [This is a checksum on the sequence] |
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10 20 30 40 50 60
MNNQRKKTAR PSFNMLKRAR NRVSTGSQLA KRFSKGLLSG QGPMKLVMAF IAFLRFLAIP
70 80 90 100 110 120
PTAGILARWG SFKKNGAIKV LRGFKKEISN MLNIMNRRKR SVTMLLMLLP TALAFHLTTR
130 140 150 160 170 180
GGEPHMIVSK QEREKSLLFK TSVGVNMCTL IAMDLGELCE DTMTYKCPRI TEAEPDDVDC
190 200 210 220 230 240
WCNATDTWVT YGTCSQTGEH RRDKRSVALA PHVGLGLETR TETWMSSEGA WKQIQRVETW
250 260 270 280 290 300
ALRHPGFTVI ALFLAHAIGT SITQKGIIFI LLMLVTPSMA MRCVGIGSRD FVEGLSGATW
310 320 330 340 350 360
VDVVLEHGSC VTTMAKDKPT LDIELLKTEV TNPAVLRKLC IEAKISNTTT DSRCPTQGEA
370 380 390 400 410 420
TLVEEQDANF VCRRTFVDRG WGNGCGLFGK GSLLTCAKFK CVTKLEGKIV QYENLKYSVI
430 440 450 460 470 480
VTVHTGDQHQ VGNETTEHGT IATITPQAPT SEIQLTDYGA LTLDCSPRTG LDFNEMVLLT
490 500 510 520 530 540
MKEKSWLVHK QWFLDLPLPW TSGASTSQET WNRQDLLVTF KTAHAKKQEV VVLGSQEGAM
550 560 570 580 590 600
HTALTGATEI QTSGTTTIFA GHLKCRLKMD KLTLKGMSYV MCTGSFKLEK EVAETQHGTV
610 620 630 640 650 660
LVQVKYEGTD APCKIPFSTQ DEKGVTQNRL ITANPIVTDK EKPVNIETEP PFGESYIVVG
670 680 690 700 710 720
AGEKALKQCW FKKGSSIGKM FEATARGARR MAILGDTAWD FGSIGGVFTS VGKLVHQVFG
730 740 750 760 770 780
TAYGVLFSGV SWTMKIGIGI LLTWLGLNSR STSLSMTCIA VGMVTLYLGV MVQADSGCVI
790 800 810 820 830 840
NWKGRELKCG SGIFVTNEVH TWTEQYKFQA DSPKRLSAAI GKAWEEGVCG IRSATRLENI
850 860 870 880 890 900
MWKQISNELN HILLENDMKF TVVVGDVVGI LAQGKKMIRP QPMEHKYSWK SWGKAKIIGA
910 920 930 940 950 960
DIQNTTFIID GPDTPECPDD QRAWNIWEVE DYGFGIFTTN IWLKLRDSYT QMCDHRLMSA
970 980 990 1000 1010 1020
AIKDSKAVHA DMGYWIESEK NETWKLARAS FIEVKTCVWP KSHTLWSNGV LESEMIIPKI
1030 1040 1050 1060 1070 1080
YGGPISQHNY RPGYFTQTAG PWHLGKLELD FDLCEGTTVV VDEHCGNRGP SLRTTTVTGK
1090 1100 1110 1120 1130 1140
IIHEWCCRSC TLPPLRFKGE DGCWYGMEIR PVKEKEENLV KSMVSAGSGE VDSFSLGLLC
1150 1160 1170 1180 1190 1200
ISIMIEEVMR SRWSRKMLMT GTLAVFLLLI MGQLTWNDLI RLCIMVGANA SDRMGMGTTY
1210 1220 1230 1240 1250 1260
LALMATFKMR PMFAVGLLFR RLTSREVLLL TIGLSLVASV ELPNSLEELG DGLAMGIMIL
1270 1280 1290 1300 1310 1320
KLLTDFQSHQ LWATLLSLTF VKTTFSLHYA WKTMAMVLSI VSLFPLCLST TSQKTTWLPV
1330 1340 1350 1360 1370 1380
LLGSLGCKPL TMFLIAENKI WGRKSWPLNE GIMAVGIVSI LLSSLLKNDV PLAGPLIAGG
1390 1400 1410 1420 1430 1440
MLIACYVISG SSADLSLEKA AEVSWEEEAE HSGASHNILV EVQDDGTMKI KDEERDDTLT
1450 1460 1470 1480 1490 1500
ILLKATLLAV SGVYPLSIPA TLFVWYFWQK KKQRSGVLWD TPSPPEVERA VLDDGIYRIM
1510 1520 1530 1540 1550 1560
QRGLLGRSQV GVGVFQDGVF HTMWHVTRGA VLMYQGKRLE PSWASVKKDL ISYGGGWRFQ
1570 1580 1590 1600 1610 1620
GSWNTGEEVQ VIAVEPGKNP KNVQTAPGTF KTPEGEVGAI ALDFKPGTSG SPIVNREGKI
1630 1640 1650 1660 1670 1680
VGLYGNGVVT TSGTYVSAIA QAKASQEGPL PEIEDEVFRK RNLTIMDLHP GSGKTRRYLP
1690 1700 1710 1720 1730 1740
AIVREAIRRN VRTLILAPTR VVASEMAEAL KGMPIRYQTT AVKSEHTGKE IVDLMCHATF
1750 1760 1770 1780 1790 1800
TMRLLSPVRV PNYNMIIMDE AHFTDPASIA RRGYISTRVG MGEAAAIFMT ATPPGSVEAF
1810 1820 1830 1840 1850 1860
PQSNAVIQDE ERDIPERSWN SGYEWITDFP GKTVWFVPSI KSGNDIANCL RKNGKRVIQL
1870 1880 1890 1900 1910 1920
SRKTFDTEYQ KTKNNDWDYV VTTDISEMGA NFRADRVIDP RRCLKPVILK DGPERVILAG
1930 1940 1950 1960 1970 1980
PMPVTVASAA QRRGRIGRNQ NKEGDQYVYM GQPLNNDEDH AHWTEAKMLL DNINTPEGII
1990 2000 2010 2020 2030 2040
PALFEPEREK SAAIDGEYRL RGEARKTFVE LMRRGDLPVW LSYKVASEGF QYSDRRWCFD
2050 2060 2070 2080 2090 2100
GERNNQVLEE NMDVEMWTKE GERKKLRPRW LDARTYSDPL ALREFKEFAA GRRSVSGDLI
2110 2120 2130 2140 2150 2160
LEIGKLPQHL TQRAQNALDN LVMLHNSEQG GRAYRHAMEE LPDTIETLML LALIAVLTGG
2170 2180 2190 2200 2210 2220
VTLFFLSGKG LGKTSIGLLC VMASSVLLWM ASVEPHWIAA SIILEFFLMV LLIPEPDRQR
2230 2240 2250 2260 2270 2280
TPQDNQLAYV VIGLLFMILT VAANEMGLLE TTKKDLGIGH VAAENHHHAT MLDVDLRPAS
2290 2300 2310 2320 2330 2340
AWTLYAVATT VITPMMRHTI ENTTANISLT AIANQAAILM GLDKGWPISK MDIGVPLLAL
2350 2360 2370 2380 2390 2400
GCYSQVNPLT LTAAVLMLVA HYAIIGPGLQ AKATREAQKR TAAGIMKNPT VDGIVAIDLD
2410 2420 2430 2440 2450 2460
PVVYDAKFEK QLGQIMLLIL CTSQILLMRT TWALCESITL ATGPLTTLWE GSPGKFWNTT
2470 2480 2490 2500 2510 2520
IAVSMANIFR GSYLAGAGLA FSLMKSLGGG RRGTGAKGKH WERNGKDRLN QLSKSEFNTY
2530 2540 2550 2560 2570 2580
KRSGIMEVDR SEAKEGLKRG ETTKHAVSRG TAKLRWFVER NLVKPEGKVI DLGCGRGGWS
2590 2600 2610 2620 2630 2640
YYCAGLKKVT EVKGYTKGGP GHEEPIPMAT YGWNLVKLYS GKDVFFTPPE KCDTLLCDIG
2650 2660 2670 2680 2690 2700
ESSPNPTIEE GRTLRVLKMV EPWLRGNQFC IKILNPYMPS VVETLEQMQR KHGGMLVRNP
2710 2720 2730 2740 2750 2760
LSRNSTHEMY WVSCGTGNIV SAVNMTSRML LNRFTMAHRK PTYERDVDLG AGTRHVAVEP
2770 2780 2790 2800 2810 2820
EVANLDIIGQ RIENIKHEHK STWHYDEDNP YKTWAYHGSY EVKPSGSASS MVNGVVKLLT
2830 2840 2850 2860 2870 2880
KPWDAIPMVT QIAMTDTTPF GQQRVFKEKV DTRTPKAKRG TAQIMEVTAR WLWGFLSRNK
2890 2900 2910 2920 2930 2940
KPRICTREEF TRKVRSNAAI GAVFVDENQW NSAKEAVEDE RFWDLVHRER ELHKQGKCAT
2950 2960 2970 2980 2990 3000
CVYNMMGKRE KKLGEFGKAK GSRAIWYMWL GARFLEFEAL GFMNEDHWFS RENSLSGVEG
3010 3020 3030 3040 3050 3060
EGLHKLGYIL RDISKIPGGN MYADDTAGWD TRITEDDLQN EAKITDIMEP EHALLATSIF
307 | |
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