ExPASy Home page| ExPASy Home page |
Site Map | Search ExPASy | Contact us | Proteomics tools |
pIcarver v3.1pIcarver is a tool to visualize theoretical distributions of peptide pI on a given pH range, and proposes a fractionation scheme that generates fractions with similar peptide frequencies.
This tool has been developed as a service of the Bioinformatics Core Facility, within a collaboration between the Proteome Informatics Group from the Swiss Institute of Bioinformatics (SIB) and the Biomedical Proteomics Research Group (BPRG).
Java Web Start technology can be used to run
pIcarver directly from the ExPASy website.
Be sure to have at least version 1.5 of Java Runtime Environment (JRE)
installed on your computer. Otherwise, you can download
the latest version from the Sun website.
It has been fully tested on Windows 2000/XP (Firefox 2.0, IE 6.0), Linux (Firefox 2.0), Mac OS X (Safari 3.0). Do not hesitate to with your configuration (OS & browser name and version) if you encountered some troubles.
NB: as first parameter of its user interface, pIcarver requires an input list of the peptides expected in the studied sample, as well as their corresponding pI. This information must be provided as a file where each line must contain three elements, the AC of a protein (or any text, excluding whitespaces), the peptide sequence and finally its pI. You may use our Compute pI/Mw tool to compute these values.
This file contains all Staphylococcus aureus (N315) peptides and can be used as an example, in order to test the program.
New features in v3.1 (7/12/07)
The pIcarver now takes the position (given in %) of pI markers with Known pI values into account in order to adjust the theoretical pH gradient provided by the supplier to the real pH gradient used for the experiment.
Features in v3.0 (16/05/07)
The pIcarver contains now 3 cutting modes, "exact", "multiple" and
the old "minimum" mode. Moreover, it provides a window to freely
compute either pI or Volume(%) for any available pH gradient.
pIcarver v2.0 (06/03/2007) : First version available.
For any remark or suggestion, please contact .
| ExPASy Home page |
Site Map | Search ExPASy | Contact us | Proteomics tools |
 | Hosted by  LNCC Brazil | | Mirror sites: | | Australia | | Canada | | China | | Korea | | Switzerland | |